Adhesion protein complexes of malaria gametocytes assemble following parasite transmission to the mosquito

作者：Simon Nina^*; Kuehn Andrea; Williamson Kim C; Pradel Gabriele

来源：Parasitology International, 2016, 65(1): 27-30.

DOI：10.1016/j.parint.2015.09.007

• Multi-protein complex
• Plasmodium falciparum
• PfCCp protein
• Pfs230
• Gametogenesis
• Transmission

During differentiation in the human, the gametocytes of the malaria parasite Plasmodium falciparum display a remarkable number of adhesive proteins on their plasma membrane. These include the PfCCp protein family of six secreted proteins that assemble to multimeric protein complexes (MPCs) within the gametocyte parasitophorous vacuole. We now show that the PfCCp-based MPCs are linked to the gametocyte plasma membrane via interactions with Pfs230, a binding-partner of the GPI-anchored Pfs48/45. Upon onset of gametogenesis, which takes place after gametocyte uptake by blood-feeding mosquitoes, GPI-anchored Pfs25 joins the MPC, providing an additional link of its components to the plasma membrane. Gametogenesis also initiates cleavage of Pfs230 at its N-terminal site, resulting in its increased interaction with the MPC. Either lack of Pfs230 or impaired Pfs230 processing causes proteolysis of the PfCCp proteins and release from the MPC Our data point to MPC assembly as a crucial step for sexual reproduction.

• 出版日期2016-2
• 单位河北医科大学