A novel method for diagnosis of smear-negative tuberculosis patients by combining a random unbiased Phi29 amplification with a specific real-time PCR

作者：Pang, Yu; Lu, Jie; Yang, Jian; Wang, Yufeng; Cohen, Chad; Ni, Xin; Zhao, Yanlin^*

来源：Tuberculosis, 2015, 95(4): 411-414.

DOI：10.1016/j.tube.2015.03.011

• Tuberculosis
• Diagnostics
• Smear negative
• REPLI real-time PCR

In this study, we develop a novel method for diagnosis of smear-negative tuberculosis patients by performing a random unbiased Phi29 amplification prior to the use of a specific real-time PCR. The limit of detection (LOD) of the conventional real-time PCR was 100 colony-forming units (CFU) of MTB genome/reaction, while the REPLI real-time PCR assay could detect 0.4 CFU/reaction. In comparison with the conventional real-time PCR, REPLI real-time PCR shows better sensitivity for the detection of smearnegative tuberculosis (P = 0.015).

• 出版日期2015-7
• 单位首都医科大学; 中国疾病预防控制中心