Gene expression changes in patients with fulminant type 1 diabetes

作者：Wang Zhen; Zheng Chao; Tan Yu-yu; Li Yi-jun; Yang Lin; Huang Gan; Lin Jian; Zhou Zhi-guang^*

来源：Chinese Medical Journal, 2011, 124(22): 3613-3617.

DOI：10.3760/cma.j.issn.0366-6999.2011.22.003

• fulminant type 1 diabetes
• peripheral blood mononuclear cells
• gene expression profile

Background Fulminant type 1 diabetes (F1D) is a complex disease. Microarray analysis was used to identify gene expression changes and obtain understanding of the underlying mechanisms. @@@ Methods Microarray analysis was performed on peripheral blood mononuclear cells from six F1D patients and six matched healthy subjects. Real-time polymerase chain reaction was used to verify the differentially expressed genes. NK cell activity was detected by methyl thiazoleterazolium assay. @@@ Results Microarray analysis identified 759 genes differing in expression between F1D patients and controls at a false discovery rate of 0.05. Expression of TLR9, ELF4 and IL1RAP were verified and consistent with changes in microarray results. NK cell activity was decreased in F1D. With use of a knowledge base, differentially expressed genes could be placed within different pathways with predicted functions including interleukin-1, and tumor necrosis factor-a signaling. @@@ Conclusions These results identify several genes indicating possible mechanisms in F1D. NK cell dysfunction resulting from changes in expression of TLR9, ELF4 and IL1RAP, and pathways of interleukin-1 and tumor necrosis factor-a signaling might be involved in F1D through inducing beta-cell dysfunction. Chin Med J 2011;124(22):3613-3617

• 出版日期2011-11-20
• 单位中南大学