Differential gene expression of gamma-actin, Toll-like receptor 2 (TLR-2) and interleukin-1 receptor-associated kinase 4 (IRAK-4) in Mya arenaria haemocytes induced by in vivo infections with two Vibrio splendidus strains

作者：Mateo Dante R^*; Greenwood Spencer J; Araya Mebrahtu T; Berthe Franck C J; Johnson Gerry R; Siah Ahmed

来源：Developmental and Comparative Immunology, 2010, 34(7): 710-714.

DOI：10.1016/j.dci.2010.02.006

• Host response
• Immune gene regulation
• Housekeeping gene
• Vibrio splendidus
• Mya arenaria
• Haemocyte

Immune function gene expression in Mya arenaria haemocytes was evaluated following in vivo infection with Vibrio splendidus LGP32-GFP and 7SHRW. Elongation factor 1 alpha (EF-1 alpha) with 2 (EF-2), after challenge with LGP32-GFP, and EF-1 alpha with the ribosomal protein S-18, after challenge with 7SHRW, were found to be the most stable housekeeping genes. Using these internal controls and comparing the regulation induced by both strains, up-regulation of gamma-actin, down-regulation of TLR-2 and up-regulation of IRAK-4 was significantly higher after challenge with LGP32-GFP (p < 0.001, p = 0.001 and p < 0.05, respectively). These results suggest specific responses at a molecular level modulated by the bacterial strains. LGP32-GFP induced marked responses which coincide with a similar trend previously found on phenotypic responses under our experimental model.

• 出版日期2010-7