科研之友
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摘要
Genetic diversity was assessed among 40 wheat genotypes consisting of new plant type (NPT) wheat derivatives, advanced breeding lines and released varieties since 1905 using 40 STMS markers. The total number of alleles amplified by 40 STMS markers was 113. The number of alleles amplified per STMS marker varied from one to six with an average of 3.32 alleles among the polymorphic markers. More alleles were detected for the B (3.55) genome derived STMS markers than A (3.18) and D (2.75) genomes. The maximum polymorphic information content (PIC) of 0.63 with an average of 0.35 was recorded while the PIC values for A, B, and D genomes were 0.243, 0.288 and 0.339 respectively. We identified 12 informative markers with three to six alleles and average of four alleles per marker and PIC values ranged from 0.31 to 0.63.
Pair wise similarity coefficients ranged from 0.22 to 0.77 suggesting wider genetic diversity among wheat genotypes. The advanced breeding lines and NPT wheat derivatives exhibited different levels of diversity as compared with released cultivars since 1905 which could be utilized in widening the genetic base of wheat and as parental lines to tag as well as to mobilize useful traits for genetic improvement of wheat.
- 出版日期2014-2
