Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester

作者：Campos Adriana^*; Vendramini Costa Debora Barbosa; Longato Giovanna Barbarini; Zermiani Tailyn; Tasca Gois Ruiz Ana Lucia; de Carvalho Joao Ernesto; Pandiella Atanasio; Cechinel Filho Valdir

来源：International Journal of Toxicology, 2016, 35(6): 666-671.

DOI：10.1177/1091581816659660

• Synadenium grantii
• antiproliferative action
• nonpolar fraction
• phorbol ester

Synadenium grantii is frequently used for the treatment of various diseases such as allergies, gastric disorders, and especially cancer. The aim of this study was to evaluate the possible antiproliferative potential of the methanol extract, fractions, and pure compounds from the stems of S grantii. Phytochemical analysis was carried out by conventional chromatographic techniques, and the antiproliferative activity was analyzed using the sulforhodamine B assay and an MTT-based assay. Nonpolar fraction and its subfractions from the stems of S grantii exhibited promising cytostatic effect against several human tumor cell lines (glioma, breast, kidney, and lung), with total grown inhibition values ranging from 0.37 to 2.9 g/mL. One of the active principles of this plant was identified as a rare phorbol diterpene ester, denoted as 3,4,12,13-tetraacetylphorbol-20-phenylacetate. This compound demonstrated antiproliferative activity against glioma, kidney, lung, and triple-negative breast cancer cell lines. These results demonstrate that S grantii stems produce active principles with relevant antiproliferative potential.

• 出版日期2016-12