科研之友
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摘要
Recent studies have focused on enantiomeric behaviors of chiral organochlorine pesticides (OCPs) in biotic matrix because they provide insights into the biotransformation processes of chiral OCPs. In the present paper, a double in-line column chromatographic method was developed to effectively remove the lipid impurity in different biotic samples for clean-up of OCPs. After an initial Soxhlet extraction of OCPs from the biotic samples by a mixture of acetone and dichloromethane (DCM), dimethyl sulfoxide (DMSO) was directly added to the extract, and low boiling point solvents (acetone and DCM) were then evaporated. OCPs remained in DMSO were eluted via column 1 filled with silicon gel, and subsequently passed through column 2 packed with 15% deactivated florisil. This novel method was characterized by significant time and solvent savings. The recovery rates of alpha-HCH (hexachlorocyclohexane), beta-HCH, gamma-HCH and delta-HCH were 78.5 +/- 3.1%, 72.4 +/- 7.7%, 72 +/- 4.0% and 70.0 +/- 8.7%, respectively, and 92.5 +/- 3.8%, 79.7 +/- 6.7% and 83.4 +/- 6.5% for 1,1-dichloro-2-(2-chlorophenyl)-2-(4-chlorophenyl) ethylene (o, p'-DDE), 1,1-dichloro-2-(2-chlorophenyl)2-(4-chloro phenyl)ethane (o, p'-DDD) and 1,1,1-trichloro-2-(2-chlorophenyl)-2-(4-chlorophenyl) ethane (o, p'-DDT), separately. In addition, the separation efficiencies of the target compounds by both achiral and chiral gas chromatographic columns were satisfactory using the established method. Therefore, the double in-line column chromatography was a useful alternative method for pretreatment of OCPs in different biotic samples.
- 出版日期2008-5
- 单位北京大学
