Murine erythroid 5-aminolevulinate synthase: Truncation of a disordered N-terminal extension is not detrimental for catalysis

作者：Stojanovski Bosko M^*; Breydo Leonid; Uversky Vladimir N; Ferreira Gloria C^*

来源：Biochimica et Biophysica Acta-Proteins and Proteomics, 2016, 1864(5): 441-452.

DOI：10.1016/j.bbapap.2016.02.002

• 5-Aminolevulinate synthase
• Pyridoxal phosphate
• Heme
• Intrinsically disordered proteins
• Sideroblastic anemia
• Porphyria
• Mitochondrial processing

5-Aminolevulinate synthase (ALAS), a pyridoxal 5'-phosphate (PLP)-dependent homodimeric enzyme, catalyzes the initial step of heme biosynthesis in non-plant eukaryotes. The precursor form of the enzyme is translated in the cytosol, and upon mitochondrial import, the N-terminal targeting presequence is proteolytically cleaved to generate mature ALAS. In bone marrow-derived erythroid cells, a mitochondrial- and site-specific endoprotease of yet unknown primary structure, produces a protein shorter than mature erythroid ALAS (ALAS2) found in peripheral blood erythroid cells. This truncated ALAS2 lacks the presequence and the N-terminal sequence (corresponding to similar to 7 KDa molecular mass) present in ALAS2 from peripheral blood erythroid cells. How the truncation affects the structural topology and catalytic properties of ALAS2 is presently not known. To address this question, we created a recombinant, truncated, murine ALAS2 (Delta mALAS2) devoid of the cleavable N-terminal region and examined its catalytic and biophysical properties. The N-terminal truncation of mALAS2 did not significantly affect the organization of the secondary structure, but a subtle reduction in the rigidity of the tertiary structure was noted. Furthermore, thermal denaturation studies revealed a decrease of 4.3 degrees C in the T-m, value of Delta mALAS2, implicating lower thermal stability. While the k(cat) of Delta mALAS2 is slightly increased over that of the wild-type enzyme, the slowest step in the Delta mALAS2-catalyzed reaction remains dominated by ALA release. Importantly, intrinsic disorder algorithms imply that the N-terminal region of mALAS2 is highly disordered, and thus susceptible to proteolysis. We propose that the N-terminal truncation offers a cell-specific ALAS2 regulatory mechanism without hindering heme synthesis.

• 出版日期2016-5