Annotating genomes with massive-scale RNA sequencing

作者:Denoeud France; Aury Jean Marc*; Da Silva Corinne; Noel Benjamin; Rogier Odile; Delledonne Massimo; Morgante Michele; Valle Giorgio; Wincker Patrick; Scarpelli Claude; Jaillon Olivier; Artiguenave Francois
来源:Genome Biology, 2008, 9(12): R175.
DOI:10.1186/gb-2008-9-12-r175

摘要

Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.

  • 出版日期2008
  • 单位中国地震局