Application of modified pyrolytic graphite electrode as a sensor in the simultaneous assay of adenine and adenosine monophosphate

作者：Goyal Rajendra N^*; Chatterjee Sanghamitra; Rana Anoop Raj Singh; Chasta Himanshu

来源：Sensors and Actuators B: Chemical , 2011, 156(1): 198-203.

DOI：10.1016/j.snb.2011.04.017

• Single-walled carbon nanotubes
• Adenine
• Adenosine-5 '-monophosphate
• Square wave voltammetry
• Pyrolytic graphite electrode

A simple and sensitive method based on square wave voltammetry (SWV) at single-walled carbon nanotube (SWNT) modified edge plane pyrolytic graphite electrode (EPPGE) is proposed for the simultaneous determination of adenine and adenosine-5'-monophosphate (5'-AMP). The modified electrode exhibits remarkable electrocatalytic properties towards adenine and 5'-AMP oxidation with a peak potential of similar to 850 and 1165 mV respectively. Linear calibration curves are obtained over the concentration range of 5-100 nM for adenine and 10-100 nM for 5'-AMP with sensitivity of 677 and 476 nA nM(-1) for adenine and 5'-AMP respectively. The limit of detection for adenine and 5'-AMP was found to be 37 x 10(-10) M and 76 x 10(-10) M, respectively. The effect of pH revealed that the oxidation of adenine and 5'-AMP at SWNT modified EPPGE involved equal number of electrons and protons. The modified electrode exhibited high stability and reproducibility.

• 出版日期2011-8-10