Two-dimensional electrophoresis on a microfluidic chip for quantitative amino acid analysis

作者：Xu Bo; Feng Xiaojun; Xu Youzhi; Du Wei; Luo Qingming; Liu Bi Feng^*

来源：Analytical and Bioanalytical Chemistry, 2009, 394(7): 1911-1917.

DOI：10.1007/s00216-009-2860-3

• Microfluidic chip
• Two-dimensional electrophoresis
• Micellar electrokinetic chromatography
• Capillary zone electrophoresis

Analysis of complex biological samples requires the use of high-throughput analytical tools. In this work, a microfluidic two-dimensional electrophoresis system was developed with mercury-lamp-induced fluorescence detection. Mixtures of 20 standard amino acids were used to evaluate the separation performance of the system. After fluorescent labeling with fluorescein isothiocyanate, mixtures of amino acids were separated by micellar electrokinetic chromatography in the first dimension and by capillary zone electrophoresis in the second. A double electrokinetic valve system was employed for the sample injection and the switching between separation channels. Under the optimized conditions, 20 standard amino acids were effectively separated within 20 min with high resolution and repeatability. Quantitative analysis revealed linear dynamic ranges of over three orders of magnitudes with detection limits at micromolar range. To further evaluate the reliability of the system, quantitative analysis of a commercial nutrition supplement liquid was successfully demonstrated.

• 出版日期2009-8
• 单位华中科技大学