诱变选育D-泛解酸内酯水解酶高产菌及发酵条件优化

作者：Shen Zhiqiang; Xing Ben; Ding Zhendong; Yang Jingwen; Hu Xueqin; Zhang Hongbin^*

来源：Food and Fermentation Industries, 2022, 48(23): 42-48.

DOI：10.13995/j.cnki.11-1802/ts.031194

• ARTP mutagenesis
• D-lactonohydrolase
• Fusarium
• optimization of fermentation conditions
• UV-LiCl mutagenesis

A Fusarium strain with pantothenic acid lactone hydrolase activity was selected as the starting strain. A strain with high activity of D-lactonohydrolase was screened by four rounds of progressive mutagenesis with atmospheric room temperature plasma (ARTP) and UV-LiCl technology. The optimum treatment time of ARTP and UV-LiCl mutation was 80 s and the concentration of UiCl was 0. 6%). A strain of 4-80-6 with an enzyme activity of 3. 46 U/mL was finally screened through preliminary screening of color changing circle and secondary screening of shaking flask. The enzyme activity was 143. 66% higher than that of the original strain. After eight generations, the heritability of the strain was relatively stable. When the substrate concentration was 20%, the hydrolysis rate of mutant strain of 4-80-6 increased from 11.6% to 17. 1%, and the optical purity increased from 93. 5% to 98. 3%. The fermentation conditions of mutant strain of 4-80-6 were optimized. The optimum conditions for enzyme production were as follows; the culture temperature was 25 XI, the initial pH of culture medium was 8. 5, the inoculation amount was 10. 5% and the culture time was 48 h. Under these conditions, the enzyme activity was 4. 33 U/mL, which was 25. 14% higher than that before optimization. The results provide an effective strategy for the industrial application of DL panthenolide enzymatic resolution. ? 2022 China National Research Institute of Food and Fermentation.

• 出版日期2022
• 单位合肥工业大学