Distribution of Ribonucleoprotein and Protein-Only RNase P in Eukarya

作者：Lechner Marcus; Rossmanith Walter; Hartmann Roland K; Thoelken Clemens; Gutmann Bernard; Giege Philippe; Gobert Anthony

来源：Molecular Biology and Evolution, 2015, 32(12): 3186-3193.

DOI：10.1093/molbev/msv187

• RNase P
• eukaryal evolution
• PRORP
• ribonucleoproteins
• tRNA biogenesis

RNase P is the endonuclease that removes 50 leader sequences from tRNA precursors. In Eukarya, separate RNase P activities exist in the nucleus and mitochondria/plastids. Although all RNase P enzymes catalyze the same reaction, the different architectures found in Eukarya range from ribonucleoprotein (RNP) enzymes with a catalytic RNA and up to 10 protein subunits to single-subunit protein-only RNase P (PRORP) enzymes. Here, analysis of the phylogenetic distribution of RNP and PRORP enzymes in Eukarya revealed 1) a wealth of novel P RNAs in previously unexplored phylogenetic branches and 2) that PRORP enzymes are more widespread than previously appreciated, found in four of the five eukaryal supergroups, in the nuclei and/or organelles. Intriguingly, the occurrence of RNP RNase P and PRORP seems mutually exclusive in genetic compartments of modern Eukarya. Our comparative analysis provides a global picture of the evolution and diversification of RNase P throughout Eukarya.

• 出版日期2015-12