Application of preparative high-speed counter-current chromatography for isolation and separation of schizandrin and gomisin A from Schisandra chinensis

作者：Peng JY; Fan GR^*; Qu LP; Zhou X; Wu YT

来源：Journal of Chromatography A, 2005, 1082(2): 203-207.

DOI：10.1016/j.chroma.2005.05.091

• preparative chromatography
• counter-current chromatography
• schizandrin
• gomisin A
• Schisandra chinensis

Following an initial cleaning-up step on the D 101 macroporous resin, a preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.9:0.9:1, v/v) was used to isolate and separate schizandrin and gomisin A from Schisandra chinensis. A total of 107 mg schizandrin and 36 mg gomisin A with purities of 99.5% and 99.1% were obtained from 400 mg crude extract in one-step elution and less than 3 h, and the structure identification was performed by UV, IR, MS, H-1 NMR and C-13 NMR.

• 出版日期2005-8-5
• 单位中国人民解放军第二军医大学