Hyaluronic acid signals for repair in ethanol-induced apoptosis in skin cells in vitro

作者：Neuman Manuela G^*; Oruna Loida; Coto Gabriel; Lago Guillermo; Nanau Radu; Vincent Marc

来源：Clinical Biochemistry, 2010, 43(10-11): 822-826.

DOI：10.1016/j.clinbiochem.2010.04.005

• Apoptosis
• A431 epidermoid skin cells
• Mouse fibroblasts
• Interferon alpha
• Tumor necrosis factor alpha
• Ethanol
• Hyaluronic acid
• In vitro models
• Transmission electron microscopy

Ethanol is commonly used in cosmetic and pharmaceutical preparations.
Objective: The present study aimed to assess ethanol-induced apoptosis and the possible repair by hyaluronic acid (HA) in vitro. In addition we aimed to determine the modulation of tumor necrosis factor (TNF-alpha) and interferon (IFN-alpha).
Design and methods: We treated human A431 epidermoid skin cells and mouse fibroblasts with two concentrations of ethanol for 24 h. HA obtained from umbilical cord excision was used at three concentration levels (2%, 4% and 8%) to determine its efficacy in the treatment. We measured cytotoxicity, TNF-alpha and IFN-alpha and visualized the cultures by electron microscopy.
Results: Treatment of cells with ethanol at 50 mM and 100 mM increased both the percentage of cells undergoing apoptosis, as well as the release of TNF-alpha into the culture medium.
Conclusions: Ethanol may induce apoptosis in skin cells by enhancing the effects of INF-alpha. HA in the 2% and 4% concentrations reduced TNF-alpha and morphological inflammation both in human A431 epidermoid skin cells and in mouse fibroblasts.

• 出版日期2010-7