Acetate accumulation through alternative metabolic pathways in ackA(-) pta(-) poxB(-) triple mutant in E. coli B (BL21)

作者：Phue Je Nie; Lee Sang Jun; Kaufman Jeanne B; Negrete Alejandro; Shiloach Joseph^*

来源：Biotechnology Letters, 2010, 32(12): 1897-1903.

DOI：10.1007/s10529-010-0369-7

• E. coli B (BL21) mutant
• Acetate excretion
• High glucose

Individual deletions of acs and aceA genes in E. coli B (BL21) showed little difference in the metabolite accumulation patterns but deletion of the ackA gene alone or together with pta showed acetic acid gradually accumulated to 3.1 and 1.7 g/l, respectively, with a minimal extended lag in bacterial growth and a higher pyruvate formation. Single poxB deletion in E. coli B (BL21) or additional poxB deletion in the ackA-pta mutants did not change the acetate accumulation pattern. When the acetate production genes (ackA-pta-poxB) were deleted in E. coli B (BL21) acetate still accumulated. This may be an indication that perhaps acetate is not only a by-product of carbon metabolism; it is possible that acetate plays also a role in other cellular metabolite pathways. It is likely that there are alternative acetate production pathways.

• 出版日期2010-12