Activation of TAK1 by Sef-S induces apoptosis in 293T cells

作者：Sun Xiaojun; Wang Yinyin; Yang Shigao; Ren Fangli; Xia Yongjing; Chang Zhijie^*

来源：Cellular Signalling, 2013, 25(10): 2039-2046.

DOI：10.1016/j.cellsig.2013.06.006

• Sef
• Erk1/2
• JNK
• p38
• MAPK
• Apoptosis

Sef (similar expression to fgf genes, also named IL-17RD) was identified as a negative regulator of fibroblast growth factor signaling. Sef-S, an alternative splice isoform of Set, inhibits FGF-induced NIH3T3 cell proliferation. Here we report that Sef-S physically interacts with TAK1, induces Lys63-linked TAK1 polyubiquitination on lysine 209 and TAK1-mediated JNK and p38 activation. Co-overexpression of TAK1 WT, K34R, K150R, K158R mutants with Sef-S induces Lys63-linked TAK1 polyubiquitination whereas TAK1 K63R and K209R mutants fail. Furthermore, co-overexpression of Sef-S and TAK1 induce 2931 cells apoptosis. These results reveal Sef-S actives Lys63-linked TAK1 polyubiquitination on lysine 209, induces TAK1-mediated JNK and p38 activation and also results apoptosis in 293T cells.

• 出版日期2013-10
• 单位清华大学