The effects of pre-emptive low-dose X-ray irradiation on MIA induced inflammatory pain in rats

作者：Hahm Suk Chan^*; Lee Go Eun; Kim Eun Hye; Kim Junesun; Lee Taewoong; Lee Wonho

来源：Journal of the Korean Physical Society, 2013, 63(2): 269-275.

DOI：10.3938/jkps.63.269

• Inflammatory pain
• Osteoarthritis
• X-radiation
• iNOS
• Spontaneous pain
• Allodynia
• Thermal hyperadesia

This study was performed to determine the effect of pre-emptive low-dose irradiation on the development of inflammatory pain and to characterize the potential mechanisms underlying this effect in osteoarthritis (OA) animal model. Whole-body X-irradiations with 0.1, 0.5, 1 Gy or sham irradiations were performed for 3 days before the induction of ostearthritis with monosodium iodoacetate (MIA) (40 A mu l, in saline) into the right knee joint in male Sprague Dawley rats. Behavioral tests for arthritic pain including evoked and non-evoked pain were conducted before and after MIA injection and inducible nitric-oxide synthase (iNOS) expression level was measured by western blot. Low-dose radiation significantly prevented the development of mechanical allodynia and thermal hyperalgesia and reduction in weight bearing that is regarded as a behavioral signs of non-evoked pain following MIA injection. Low-dose radiation significantly inhibited the increase in iNOS expression after MIA injection in spinal L3-5 segments in rat. %26lt;br%26gt;These data suggest that low-dose X-irradiation is able to prevent the development of arthritic pain through modulation of iNOS expression in the spinal cord dorsal horn. Thus, low-dose radiotherapy could be substituted in part for treatment with drugs for patients with chronic inflammatory disease in clinical setting.

• 出版日期2013-7