Anti-proliferative effect of a compound isolated from Cassia auriculata against human colon cancer cell line HCT 15

作者：Esakkirajan M; Prabhu N M^*; Arulvasu C; Beulaja M; Manikandan R; Thiagarajan R; Govindaraju K; Prabhu D; Dinesh D; Babu G; Dhanasekaran G

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2014, 120: 462-466.

DOI：10.1016/j.saa.2013.09.102

• Cassia auriculata
• Anti-proliferative
• HCT 15 cell line

The compound was isolated from leaves of Cassia auriculata and its structure was characterized using high-performance liquid chromatography (HPLC), liquid chromatography mass spectrometry (LC-MS), UV-vis spectroscopy (UV-vis), fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, cytotoxicity, nuclear morphology and lactate dehydrogenase assay of isolated compound was tested against human colon cancer cell line HCT 15. The isolated compound, 4-(4-chlorobenzyl)-2,3,4,5,6,7-hexahydro-7-(2-ethoxyphenyl)benzo[h][1,4,7]triazecin-8(1H)-one at 25 mu g/ml concentration and by 48 h showed 50% inhibition of human colon cancer cells (HCT 15). The results suggest that isolated compound from C auriculata has potential to prevent colon cancer cell line.

• 出版日期2014-2-24