摘要

A new amperometric method for antioxidant activity (AOA) evaluation, based on a Prussian Blue (PB)-modified graphite electrode (G/PB) for H2O2 detection, was proposed. Taking into account that some antioxidants act as H2O2 scavengers, the AOA was estimated amperometrically by the decrease of H2O2 concentration, and was expressed as the equivalent ascorbic acid (AA) concentration, chosen as a reference antioxidant. The present study describes the preparation and characterization of the H2O2 sensor, as well as its use for AOA evaluation in real samples. The electroanalytical parameters obtained for both H2O2 and ascorbic acid (AA) determinations, the sensor stability and the good agreement existing between our results and those already published proved that the proposed approach may be successfully used as a simple and reliable method for AOA evaluation.

  • 出版日期2010-12