ATP Photosynthetic vesicles for light-driven bioprocesses

作者：Hara Kiyotaka Y; Suzuki Rie; Suzuki Toshiharu; Yoshida Masasuke; Kino Kuniki^*

来源：Biotechnology Letters, 2011, 33(6): 1133-1138.

DOI：10.1007/s10529-011-0544-5

• ATP synthase
• Membrane vesicle
• Photosynthetic ATP synthesis
• Rhodopsin
• Vesicles

We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H ? transporter) and TF(0)F(1)-ATP synthase (a thermostable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATP-regenerating bioprocess for various ATP-hydrolyzing bioproductions.

• 出版日期2011-6