Monitoring an enzyme purification process using on-line and in-line NIR measurements

作者：Klimkiewicz Anna^*; Mortensen Peter Paasch; Zachariassen Christian B; van den Berg Frans W J

来源：Chemometrics and Intelligent Laboratory Systems, 2014, 132: 30-38.

DOI：10.1016/j.chemolab2014.01.002

• On-line NIR
• In-line NIR
• Enzyme activity
• Ultrafiltration
• Process monitoring
• Real-time

The key parameter during purification of enzymes is the strength of the intermediates and products. Employing near infrared spectroscopy, enzyme activity of the concentrate can be obtained much faster than by traditional off-line analysis in a central laboratory. This paper describes the development of a monitoring system for the enzyme activity in a recovery plant and provides a comparison between two real-time probe setups (on-line v. in-line) in a full scale application. The focus in the paper will be on the chemometric calibration development plus validation and the application of the models for monitoring purposes. For this investigation, four different types of industrial enzymes have been sampled over a period of ten months. Real-time output of the partial least squares regression models was used along with conventional process data generated to evaluate the pros and cons of the in-line and on-line setup. Both implementations deliver good results in monitoring the ultrafiltration process, but problems (precipitation/phase transition) were occasionally encountered for the on-line arrangement.

• 出版日期2014-3-15