Multispectral studies of DNA binding, antioxidant and cytotoxic activities of a new pyranochromene derivative

作者：Dehkordi Mahvash Farajzadeh; Dehghan Gholamreza^*; Mahdavi Majid; Feizi Mohammad Ali Hosseinpour

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2015, 145: 353-359.

DOI：10.1016/j.saa.2015.03.026

• Pyranochromene
• Outside DNA binding
• Cytotoxicity
• Antioxidant potential

The binding properties of a new pyranochromene derivative, 2-amino-4-(3-hydroxyphenyl)-5-oxo-4H, 5H-pyrano-[3, 2-c] chromene-3-carbonitrile (3-HC) with calf thymus DNA (ctDNA) have been investigated by UV-vis absorption, circular dichroism, fluorescence spectroscopy and viscosity measurement. These results indicated that 3-HC can interact with DNA through non-intercalative mode and the intrinsic binding constant (K-b) for 3-I-1C with DNA was estimated to be 3.6 x 10(3) M-1. The antioxidant activity experiments show that 3-HC also exhibit good antioxidant activity in DPPH free radical scavenging and ferric reducing ability methods. Moreover, 3-HC exhibited cytotoxic activity against K562, human chronic myelogenous leukemia cells, with IC50 value of 146 mu M and the cells responded to the treatment with mostly through apoptosis.

• 出版日期2015-6-15