Anti-PRRSV effect and mechanism of sodium tanshinone IIA sulfonate in vitro

作者：Sun, Na; Zhao, Xin; Bai, Xi-Yun; Niu, Li; Song, Mei-Qin; Sun, Yao-Gui; Jiang, Jun-Bing; Li, Hong-Quan^*

来源：Journal of Asian Natural Products Research, 2012, 14(8): 721-728.

DOI：10.1080/10286020.2012.685727

• sodium tanshinone IIA sulfonate
• anti-PRRSV
• Marc-145
• CPE
• MTT

This experiment was conducted to study the antiviral activities of sodium tanshinone IIA sulfonate (STS) against porcine reproductive and respiratory syndrome virus (PRRSV) and its mechanism. Anti-PRRSV activities of STS were observed on Marc-145 cells by using visualization of cytopathologic effect assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, and its antiviral mechanism was determined by time-of-addition assay, adsorption inhibition assay, and virucidal assay. The results showed that STS could reduce the damage of PRRSV to Marc-145 cells, with the inhibition ratio exceeding to 100%, at the maximum noncytotoxic concentration. The time-of-addition and virucidal assays indicated that the anti-PRRSV activities of STS could be due to inhibiting the virus replication or/and inactivating the virus directly. The inhibition of the virus attachment was not discovered in adsorption inhibition assay. The results proved that STS had strong antiPRRSV activity and encouraged for further exploration of STS.

• 出版日期2012
• 单位山西农业大学