Differentiation of human bone marrow -derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells

作者：Yang Ling Ling; Zhou Qing Jun; Wang Yao; Wang Yi Qiang

来源：International Journal of Ophthalmology, 2010, 3(1): 23-27.

• bone marrow-derived mesenchymal stem cells
• retinal pigment epithelial cells
• neural-like cells
• bovine retinal extract

AIM: To detect the differentiation effects of retinal cells or extracts on bone marrow-derived mesenchymal stem cells (BMSC).
METHODS: Human fetal BMSC were previously labelled by carboxyfluorescein succinimidyl ester (CFSE), and co-cultured with retinal pigment epithelial (RPE) cells which were pre-treated with ultraviolet irradiation at a ratio of 1:1 to induce the differentiation of BMSC for up to 14 days. In some assays, a retinal extract of bovine retinal extract (BRE) was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition, Neuron-specific enolase (NSE), Nestin and Glial fibrillary acidic protein (GFAP) immunostaining were performed to determine the characteristics of BMSC.
RESULTS: The results indicated that by co-cultured with RPE cells, fetal BMSC were differentiated into neural-like cells expressing special neuronal markers Nestin, GFAP and NSE. And the expression of these markers was obviously increased by BRE.
CONCLUSION: Retina derived cells and extracts can induce the differentiation of BMSC into neural-like cells.

• 出版日期2010-3-18
• 单位山东省眼科研究所