Media optimization for elevated molecular weight and mass production of pigment-free pullulan

作者：Yu Xiaoliu; Wang Yulei; Wei Gongyuan^*; Dong Yingying

来源：Carbohydrate Polymers, 2012, 89(3): 928-934.

DOI：10.1016/j.carbpol.2012.04.038

• Pullulan
• Molecular weight
• Orthogonal array design
• Response surface methodology
• Aureobasidium pullulans

In this study, an Aureobasidium pullulans SZU 1001 mutant, deficient in pigment production, was screened by complex UV and gamma-ray mutagenesis. Medium composition optimization for increased pullulan molecular weight and production was conducted using this mutant. Six nutrients: yeast extract, (NH4)(2)SO4, K-2 HPO4, NaCl, MgSO4 center dot 7H(2)O and CaCl2 were detected within pullulan production in flasks. It is shown that NaCl and K-2 HPO4 have significant influences on molecular weight of pullulan, while yeast extract and (NH4)(2)SO4 significantly affect pullulan yield. To achieve a higher molecular weight and more efficient pullulan production, a response surface methodology approach was introduced to predict an optimal nutrient combination. A molecular weight of 5.74 x 10(6) and pullulan yield on glucose of 51.30% were obtained under batch pullulan fermentation with the optimized media, which increased molecular weight and pullulan production by 97.25% and 11.04%, respectively compared with the control media.

• 出版日期2012-7-1
• 单位苏州大学