Measuring salivary analytes from free-ranging monkeys

作者：Higham James P^*; Vitale Alison B; Rivera Adaris Mas; Ayala James E; Maestripieri Dario

来源：Physiology & Behavior, 2010, 101(5): 601-607.

DOI：10.1016/j.physbeh.2010.09.003

• Field methods
• Laboratory methods
• Enzyme immuno-assay
• Immune function
• Cortisol
• Alpha amylase
• Secretory immunoglobulin A
• Saliva
• Mammalian

Studies of large free-ranging mammals have been revolutionized by non-Invasive methods for assessing physiology which usually involve the measurement of fecal or urinary biomarkers However such techniques are limited by numerous factors To expand the range of physiological variables measurable non-invasively from free-ranging primates we developed techniques for sampling monkey saliva by offering monkeys ropes with oral swabs sewn on the ends We evaluated different attractants for encouraging individuals to offer samples and proportions of individuals in different age/sex categories willing to give samples We tested the saliva samples we obtained in three commercially available assays cortisol salivary alpha amylase and secretory immunoglobulin A We show that habituated free-ranging rhesus macaques will give saliva samples voluntarily without training with 100% of Infants and over 50% of adults willing to chew on collection devices Our field methods are robust even for analytes that show poor recovery from cotton and/or that have concentrations dependent on salivary flow rate We validated the cortisol and SAA assays for use in rhesus macaques by showing aspects of analytical validation such as that samples dilute linearly and in parallel to assay standards We also found that values measured correlated with biologically meaningful characteristics of sampled individuals (age and dominance rank) The SIgA assay tested did not react to samples Given the wide range of analytes measurable in saliva but not in feces or urine our methods considerably impr

• 出版日期2010-12-2