Adeno-associated virus serotype 2 mediated transduction and coexpression of the human apoAI and SR-BI gene in HepG2 cells

作者：Li, Bingnan; Zhang, Juan; Li, Zhiyan; Tan, Mengqun^*

来源：Molecular Biology Reports, 2012, 39(1): 25-32.

DOI：10.1007/s11033-011-0706-5

• Adeno-associated virus
• Gene transfer
• Apolipoprotein A-I
• Scavenger receptor class B type I
• Reverse cholesterol transport (RCT)
• Expression

Cholesterol efflux is the first step in the reverse cholesterol transport (RCT) pathway, removing excess cholesterol from tissues, including the arterial wall, thus preventing the development of atherosclerosis. Adeno-associated virus (rAAV) has demonstrated significant promise as a DNA-delivery vector to treat serious human diseases. In this study, we constructed recombinant adeno-associated viruses coexpressing apoAI and SR-BI successfully, the double gene mRNA and protein were both strongly expressed in transduced HepG2 cells. A novel safe and efficient method of promoting the reverse cholesterol transport (RCT) may be established. These results may provide a new method for gene therapy of Arteriosclerosis.

• 出版日期2012-1
• 单位中南大学