A reactivity-based probe of the intracellular labile ferrous iron pool

作者：Spangler Benjamin; Morgan Charles W; Fontaine Shaun D; Vander Wal Mark N; Chang Christopher J; Wells James A^*; Renslo Adam R^*

来源：Nature Chemical Biology, 2016, 12(9): 680-+.

DOI：10.1038/nchembio.2116

Improved methods for studying intracellular reactive Fe(II) are of significant interest for studies of iron metabolism and disease-relevant changes in iron homeostasis. Here we describe a highly selective reactivity-based probe in which a Fenton-type reaction with intracellular labile Fe(II) leads to unmasking of the aminonucleoside puromycin. Puromycin leaves a permanent and dose-dependent mark on treated cells that can be detected with high sensitivity and precision using a high-content, plate-based immunofluorescence assay. Using this new probe and screening approach, we detected alteration of cellular labile Fe(II) in response extracellular iron conditioning, overexpression of iron storage and/or export proteins, and post-translational regulation of iron export. We also used this new tool to demonstrate that labile Fe(II) pools are larger in cancer cells than in nontumorigenic cells.

• 出版日期2016-9