Adenovirus-mediated overexpression of cystic fibrosis transmembrane conductance regulator enhances invasiveness and motility of serous ovarian cancer cells

作者：Xu, Jiao; Lin, Liangbo; Yong, Min; Dong, Xiaojing; Yu, Tinghe; Hu, Lina^*

来源：Molecular Medicine Reports, 2016, 13(1): 265-272.

DOI：10.3892/mmr.2015.4509

• adenoviral vector
• cystic fibrosis transmembrane conductance regulator
• homologous recombination
• serous ovarian cancer
• tumor progression

The cystic fibrosis transmembrane conductance regulator (CFTR) belongs to the adenosine triphosphate-binding cassette transporter family, members of which are involved in several types of cancer. Previous studies by our group reported that CFTR was highly expressed in serous ovarian cancer (SOC) tissues, and that knockdown of CFTR suppressed the proliferation of ovarian cancer in vitro and in vivo. Thus, the aim of the present study was to construct a recombinant adenoviral vector for the expression of the human CFTR gene in order to study the role of CFTR overexpression in the malignant invasion and migration of SOC cells in vitro. The present study then focused on the mechanisms of the role of CFTR in the migratory and invasive malignant properties of SOC cells. The CFTR gene was inserted into an adenoviral vector by using the AdEasy system in order to obtain the Ad-CFTR overexpression vector, which was used to transfect the A2780 SOC cell line. Reverse-transcription polymerase chain reaction, western blot analysis and immunofluorescence were performed to detect the expression and localization of CFTR. Cell invasion and motility of the transfected cells compared with those of control cells were observed using Transwell and wound healing assays. A similar to 4,700 bp fragment of the CFTR gene was confirmed to be correctly cloned in the adenoviral vector and amplification of Ad-CFTR was observed in HEK293 cells during package. After 48 h of transfection with Ad-CFTR, similar to 90% of A2780 cells were red fluorescence protein-positive. Immunofluorescence showed that following transfection, CFTR expression was increased and CFTR was located in the cell membrane and cytoplasm. CFTR overexpression was shown to enhance the invasion and motility of A2780 cells in vitro. Furthermore, the effects of CFTR overexpression on the activation c-Src signaling were observed by western blot analysis. CFTR overexpressing cells showed the lowest activity of phospho-Src (Tyr530), suggesting that CFTR may affect the activation of c-Src signaling. The results of the present study demonstrated that adenovirus-mediated CFTR overexpression enhanced cell invasion and motility of SOC cells in vitro. Furthermore, CFTR may be critical for the activation of c-Src signaling.

• 出版日期2016-1
• 单位重庆医科大学