Anti-lipoapoptotic effects of Alisma orientalis extract on non-esterified fatty acid-induced HepG2 cells

作者：Jeong Hyeon Soo; Cho Young Hwan; Kim Kang Hoon; Kim Yumi; Kim Ki Suk; Na Yun Cheol; Park Jiyoung; Lee In Seung; Lee Jang Hoon; Jang Hyeung Jin

来源：BMC Complementary and Alternative Medicine, 2016, 16(1): 239.

DOI：10.1186/s12906-016-1181-2

• Alisma orientale
• HepG2
• Nonalcoholic fatty liver disease
• De novo lipogenesis
• Lipoapoptosis
• Inflammation

Background: Liver steatosis was caused by lipid accumulation in the liver. Alisma orientale (AO) is recognized as a promising candidate with therapeutic efficacy for the treatment of nonalcoholic fatty liver disease (NAFLD). HepG2 hepatocyte cell line is commonly used for liver disease cell model.
Method: The HepG2 cells were cultured with the NEFAs mixture (oleic and palmitic acids, 2: 1 ratio) for 24 h to induce hepatic steatosis. Then different doses of Alisma orientale extract (AOE) was treated to HepG2 for 24 h. Incubated cells were used for further experiments.
Results: The AOE showed inhibitory effects on lipid accumulation in the Oil Red O staining and Nile red staining tests with no cytotoxicity at a concentration of 300 mu g/mL. Fatty acid synthase (FASN) and acetyl-CoA carboxylase 1 (ACC1) mRNA and protein expression level were down-regulated after AOE treatment. Bcl-2 associated X protein (Bax) and c-Jun N-terminal kinase (JNK) mRNA expression level were decreased as well as p-JNK (activated form of JNK), Bax, cleaved caspase-9, caspase-3 protein expression level. Anti-apopototic B-cell lymphoma 2 (Bcl-2) protein level increased after AOE treatment. In addition, inflammatory protein expression including p-p65, p65, COX-2 and iNOS were inhibited by AOE treatment.
Conclusion: The results suggest that AOE has anti-steatosis effects that involve lipogenesis, anti-lipoapoptosis, and anti-inflammation in the NEFA-induced NAFLD pathological cell model.

• 出版日期2016-7-25