A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron

作者：Hunter Therese; Bonetta Rosalin; Sacco Anthony; Vella Marita; Sultana Paul-Michael; Trinh Chi H.; Fadia Hava B. R.; Borowski Tomasz; Garcia-Fandino Rebeca; Stockner Thomas; Hunter Gary J.^*

来源：Chemistry - A European Journal, 2018, 24(20): 5303-5308.

DOI：10.1002/chem.201704655

• enzymes
• iron
• manganese
• metalloprotein
• superoxide dismutase

We have generated a site-directed mutant of the manganese superoxide dismutase SOD-3 of C.elegans (MnSOD-3) which modifies the metal specificity of the enzyme. While wild-type MnSOD-3 functions with manganese in the active site (3600Umg(-1) of protein) it has little or no activity when iron is incorporated. However, when histidine replaces glutamine 142 in the active site, the enzyme retains 50% of its activity and becomes cambialistic for its metal cofactor exhibiting very similar specific activity with either manganese or iron.

• 出版日期2018-4-6