Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae

作者：Van Vu Ba; Takino Masako; Murata Toshiki; Nakayashiki Hitoshi^*

来源：Journal of General Plant Pathology, 2011, 77(3): 147-151.

DOI：10.1007/s10327-011-0302-5

• Magnaporthe oryzae
• Gene silencing
• Retrotransposon
• PTGS
• TGS

Here we report a novel system to induce RNA silencing in Magnaporthe oryzae using the retrotransposon MAGGY. The gene-silencing vector pSilent-MG carries a MAGGY variant with a unique cloning site in the 3' UTR region and a geneticin-resistance cassette as a selection marker. A gene-silencing assay with a GFP gene revealed that pSilent-MG vectors induced gene silencing at a practical level. In the resulting GFP-silenced transformants, GFP siRNAs accumulated and DNA methylation increased in the GFP-expressing gene, implying that the vector could induce both transcriptional and post-transcriptional gene silencing.

• 出版日期2011-5