A new micro/nanoencapsulated porphyrin formulation for PDT treatment

作者：Deda Daiana K; Uchoa Adjaci F; Carita Eduardo; Baptista Mauricio S; Toma Henrique E; Araki Koiti^*

来源：International Journal of Pharmaceutics, 2009, 376(1-2): 76-83.

DOI：10.1016/j.ijpharm.2009.04.024

• Cationic porphyrin
• Polymeric encapsulation
• PDT
• HeLa cells

The highly hydrophobic 5,10,15-triphenyl-20-(3-N-methylpyridinium-yl)porphyrin(3MMe)cationic species was synthesized, characterized and encapsulated in marine atelocollagen/xanthane gum microcapsules by the coacervation method. Further reduction in the capsule size, from several microns down to about 300-400 nm, was carried out successfully by ultrasonic processing in the presence of up to 1.6% Tween 20 surfactant, without affecting the distribution of 3MMe in the oily core. The resulting creamlike product exhibited enhanced photodynamic activity but negligible cytotoxicity towards HeLa cells. The polymeric micro/nanocapsule formulation was found to be about 4 times more phototoxic than the respective phosphatidylcholine lipidic emulsion, demonstrating high potentiality for photodynamic therapy applications.

• 出版日期2009-7-6