Determination of tocopherols and sitosterols in seeds and nuts by QuEChERS-liquid chromatography

作者：Milagros Delgado Zamarreno M; Fernandez Prieto Cristina; Bustamante Rangel Myriam; Perez Martin Lara

来源：Food Chemistry, 2016, 192: 825-830.

DOI：10.1016/j.foodchem.2015.07.083

• QuEChERS
• LC
• Tocopherols
• Phytosterols
• Nuts

In the present work a simple, reliable and affordable sample treatment method for the simultaneous analysis of tocopherols and free phytosterols in nuts was developed. Analyte extraction was carried out using the QuEChERS methodology and analyte separation and detection were accomplished using HPLC-DAD. The use of this methodology for the extraction of natural occurring substances provides advantages such as speed, simplicity and ease of use. The parameters evaluated for the validation of the method developed included the linearity of the calibration plots, the detection and quantification limits, repeatability, reproducibility and recovery. The proposed method was successfully applied to the analysis of tocopherols and free phytosterols in samples of almonds, cashew nuts, hazelnuts, peanuts, tiger nuts, sun flower seeds and pistachios.

• 出版日期2016-2-1