Antioxidant activity and low toxicity of (E)-1-(1-(methylthio)-1-(selenopheny) hept-1-en-2-yl) pyrrolidin-2-one

作者：Ineu Rafael Porto; dos Santos Matheus; do Rego Barros Olga Soares; Nogueira Cristina Wayne; Teixeira Rocha Joao Batista; Zeni Gilson; Pereira Maria Ester^*

来源：Cell Biology and Toxicology, 2012, 28(4): 213-223.

DOI：10.1007/s10565-012-9217-y

• Antioxidant
• Organoselenium
• Oxidative stress
• Reactive oxygen species
• Selenium
• Toxicity

The aim of the present study was to evaluate the potential pharmacological and toxicological properties of (E)-1-(1-(methylthio)-1-(selenopheny) hept-1-en-2-yl) pyrrolidin-2-one (compound 1), an organoselenium compound. In vitro experiments showed that compound 1 presented a reduction in the lipid peroxidation induced by Fe2+ in thiobarbituric acid-reactive species (TBARS) production, and in the generation of reactive species caused by Fe2+/malonate in DCFH-DA oxidation. The high dose (500 mg/kg) induced an increase on ALT but not on AST activity. Hepatic, but not cerebral, delta-ALA-D activity from mice treated with 500 mg/kg presented a significant inhibition. Brain catalase activity was significantly inhibited by 100 mg/kg whereas hepatic catalase activity showed a significant increase at all doses. Hepatic lipid peroxidation was diminished only at lowest dose (100 mg/kg) whereas for brain tissue, all doses induced a significant reduction in TBARS levels. Brain and liver ascorbic acid contents were increased only at highest dose of compound 1. Urea and creatinine levels were not significantly altered by treatments. This is a promising compound with antioxidant activity and low toxicity, suggesting the potential beneficial activity of compound 1 against oxidative damage in many parameters studied in rats and mice.

• 出版日期2012-8