Neuroprotective effect of Lactuca sativa on glucose/serum deprivation-induced cell death

作者：Sadeghnia Hamid Reza; Farahmand Seyed Kazem; Asadpour Elham; Rakhshandeh Hassan; Ghorbani Ahmad^*

来源：African Journal of Pharmacy and Pharmacology, 2012, 6(33): 2464-2471.

DOI：10.5897/AJPP12.085

• Lactuca sativa
• glucose/serum deprivation
• brain ischemia
• rat pheochromocytoma-derived cell line (PC12)
• mouse neuroblastoma-like cell line (N2a)

This study was planned to investigate whether Lactuca sativa is capable of protecting neurons against glucose/serum deprivation (GSD)-induced cell injury, an in vitro model of brain ischemia. Two neuronlike cells, rat pheochromocytoma-derived cell line (PC12) and mouse neuroblastoma-like cell line (N2a), were cultivated for 24 h in standard media or for 6 and 12 h in GSD condition in the absence or presence of hydro-alcoholic extract (HAE), water fraction (WF), ethyl acetate fraction (EAF) or N-butanol fraction (NBF) of L. sativa. At the end of the experiments, the cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay. The L. sativa exhibited no cytotoxicity under standard condition unless at very high concentrations. Exposure of PC12 cells to GSD condition for 6 and 12 h led to 44 and 90% decrease in cell viability, respectively. The NBF and especially EAF significantly attenuated GSD-induced cell death. Also, GSD caused a significant reduction in N2a cell viability after 6 h (28%) and 12 h (84%). On the other hand, treatment of N2a cells with EAF blocked the cytotoxic effects of GSD condition. The present study revealed that intermediate polarity fraction of L. sativa (EAF) exerts neuroprotection and has the potential to be used as a new therapeutic strategy for common neurodegenerative disorders such as stroke.

• 出版日期2012-9