Metabolic engineering of Klebsiella pneumoniae J2B for co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol: Reduction of acetate and other by-products

作者：Ko Yeounjoo; Seol Eunhee; Sekar Balaji Sundara; Kwon Seongjin; Lee Jaehyeon; Park Sunghoon^*

来源：Bioresource Technology, 2017, 244: 1096-1103.

DOI：10.1016/j.biortech.2017.08.099

• Co-production
• 3-Hydroxypropionic acid
• 1,3-Propanediol
• Klebsiella pneumoniae
• Acetate reduction

Production of 3-hydroxypropionic acid (3-HP) or 1,3-propanediol (1,3-PDO) production from glycerol is challenging due to the problems associated with cofactor regeneration, coenzyme B12 synthesis, and the instability of pathway enzymes. To address these complications, simultaneous production of 3-HP and 1,3-PDO, instead of individual production of each compound, was attempted. With over-expression of an aldehyde dehydrogenase, recombinant Klebsiella pneumoniae could co-produce 3-HP and 1,3-PDO successfully. However, the production level was unsatisfactory due to excessive accumulation of many by-products, especially acetate. To reduce acetate production, we attempted; (i) reduction of glycerol assimilation through the glycolytic pathway, (ii) increase of glycerol flow towards co-production, and (iii) variation of aeration rate. These efforts were partially beneficial in reducing acetate and improving co-production: 21 g/L of 1,3-PDO and 43 g/L of 3-HP were obtained. Excessive acetate (> 150 mM) was still produced at the end of bioreactor runs, and limited co-production efficiency.

• 出版日期2017-11