A TaqMan-based qRT-PCR assay for Senecavirus A detection in tissue samples of neonatal piglets

作者：Dall Agnol Alais Maria; Arellano Otonel Rodrigo Alejandro; Leme Raquel Arruda; Alfieri Arnaud Alcindo; Alfieri Alice Fernandes

来源：Molecular and Cellular Probes, 2017, 33: 28-31.

DOI：10.1016/j.mcp.2017.03.002

• Picornavirus infection
• Quantitative PCR
• Real-time RT-PCR
• Seneca valley virus
• Swine
• Vesicular disease

This study describes a sensitive (1.3 x 10(1) genomic copies/mu L) and specific TaqMan-based qRT-PCR assay able to detect and quantify SVA RNA in porcine biological samples. The technique represents an efficient tool for the virus diagnosis and assessment of SVA load in tissues of infected animals and for epidemiological studies.

• 出版日期2017-6