Direct detection of genomic DNA with fluidic force discrimination assays

作者：Mulvaney S P^*; Ibe C N; Tamanaha C R; Whitman L J

来源：Analytical Biochemistry, 2009, 392(2): 139-144.

DOI：10.1016/j.ab.2009.05.042

• Genomic DNA
• Fluidic force discrimination (FFD)
• Biosensor
• Magnetic bead
• Fragmentation by sonication
• Bacillus anthracis
• Bacillus thuringiensis

Herein, we describe the direct detection of genomic DNA using fluidic force discrimination (FFD) assays. Starting with extracted bacterial DNA, samples are fragmented by restriction enzymes or sonication, then thermocycled in the presence of blocking and labeling sequences, and finally detected with microbead-based FFD assays. Both strain and species identification of extracted Bacillus DNA have been demonstrated in <30 min, without amplification (e.g., PCR). Femtomolar assays can be achieved with this rapid and simple procedure.

• 出版日期2009-9-15