Bone marrow derived mononuclear stromal cells and experimental model of deep endometriosis

作者：do Amaral Vivian Ferreira^*; Maestrelli Priscila de Carvalho; Francisco Julio Cesar; Guarita Souza Luiz Cesar; Kondo William; Noronha Lucia; do Amaral Thayana Ferreira

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(2): 1116-1126.

• Endometriosis
• mononuclear bone marrow stromal cells
• metalloproteinases
• transforming growth factor-beta
• experimental model

Objective: To evaluate the effect of the treatment with mononuclear bone marrow stromal cells and the expression of metalloproteinases MMP-1, MMP-9 and the factor TGF-beta 1 in Wistar rats submitted to induction of deep/retrocervical endometriosis. Methods: A total of 120 female Wistar rats were induced to a deep endometriosis and after development of the disease, the animals were randomized into three different groups, referring to the treatment applied: saline (n = 38), leuprolide (n = 30) and stem cells (n = 35). Results: There was a significant difference in reduction of implant in the stem cell (P = 0.017) and leuprolide (P = 0.025) groups. However, there was no significant difference in the saline solution group (P = 0.771). In metalloproteinases expression there was no significant difference among groups. Conclusions: Treatment with bone marrow derived-mononuclear stromal cells was effective in reducing ectopic implant area. However, there were no significant differences between groups in relation to the expression of MMP-1, MMP-9 and TGF-beta 1.

• 出版日期2016