Antifungal secondary metabolites from endophytic Verticillium sp.

作者：You Fei; Han Ting; Wu Jing zhong; Huang Bao kang; Qin Lu ping^*

来源：Biochemical Systematics and Ecology, 2009, 37(3): 162-165.

DOI：10.1016/j.bse.2009.03.008

• Endophyte
• Antibiotic
• Inhibition
• Metabolite
• Verticillium sp.

Endophytes were isolated from roots of wild Rehmannia glutinosa to screen the strains with antifungal metabolites. A strain identified as Verticillium sp. was selected for chemical and biological investigations because of the strong antifungal activity of the crude extract against Pyricularia oryzae P-2b. Chemical investigations of culture broth afforded three compounds: 2,6-dihydroxy-2-methyl-7-(prop-1E-enyl)-1-benzofuran-3(2H)-one. massariphenone and ergosterol peroxide. The metabolites were isolated by silica gel and Sephadex LH-20, 2,6-dihydroxy-2-methyl-7-(prop-1E-enyl)-1-benzofuran-3(2H)-one was reported for the first time and the chemical structure was established following the analysis of NMR, UV, IR, MS data. 2,6-Dihydroxy-2-methyl-7-(prop-1E-enyl)-1-benzofuran-3(2H)-one and ergosterol peroxide displayed clear inhibition of the growth of three pathogens as well as Verticillium sp.

• 出版日期2009-7
• 单位中国人民解放军第二军医大学