A Pair of New Antioxidant Phenolic Acid Stereoisomers Isolated from Danshen Injection (Lyophilized Powder)

作者：Liu Jing; Zhao Jianfeng; Dai Zhong; Lin Ruichao^*; Wang Gangli; Ma Shuangcheng

来源：Molecules, 2014, 19(2): 1786-1794.

DOI：10.3390/molecules19021786

• Danshen Injection
• phenolic acid
• stereoisomer
• antioxidant activity

A pair of new phenolic acid stereoisomers, (R)-norsalvianolic acid L (1) and (S)-norsalvianolic acid L (2), was isolated from the Danshen Injection (lyophilized powder). The structural elucidation and stereochemistry determination were achieved by spectroscopic and chemical methods including 1D, 2D NMR (H-1-H-1 COSY, HSQC and HMBC) and circular dichroism experiments. Their antioxidant activities were assessed by the DPPH center dot and ABTS(center dot+) scavenging methods in vitro with microplate assay. The IC50 values of 1 were 6.9 and 9.7 mu M respectively, which was close to the control salvianolic acid B (7.8 and 7.1 mu M respectively), while the IC50 values of isomer 2 were 27.1 and 25.3 mu M, respectively.

• 出版日期2014-2