Autophagy protects podocytes from sublytic complement induced injury

作者：Lv, Qianying; Yang, Fengjie; Chen, Kun; Zhang, Yu^*

来源：Experimental Cell Research, 2016, 341(2): 132-138.

DOI：10.1016/j.yexcr.2016.02.009

• Autophagy
• Podocyte
• Sublytic C5b-9
• Complement

Podocyte injury induced by sublytic complement attack is the main feature of membranous nephropathy (MN). This study aimed at investigating the impact of sublytic complement attack-related autophagy on podocyte injury in vitro. Here, we show that sublytic complement attack enhances MPC5 podocyte autophagy in vitro. Inhibition of autophagy by treatment with 3-methyladenine (3-MA) significantly increased sublytic complement attack-induced changes in the injury-related morphology, stress fiber, and podocyte apoptosis, but decreased the survival and adhesion of MPC5 podocytes. In contrast, promotion of autophagy by treatment with rapamycin mitigated sublytic complement attack-induced changes in the injury-related morphology, stress fiber, and podocyte apoptosis, but increased the survival and adhesion of MPC5 podocytes. These data suggest that autophagy may protect podocytes from sublytic complement attack-induced injury in vitro.

• 出版日期2016-2-15
• 单位华中科技大学