Embryonic alcohol exposure promotes long-term effects on cerebral glutamate transport of adult zebrafish

作者：Baggio Suelen; Mussulini Ben Hur; de Oliveira Diogo Losch; Zenki Kamila Cagliari; da Silva Emerson Santos; Rico Eduardo Pacheco^*

来源：Neuroscience Letters, 2017, 636: 265-269.

DOI：10.1016/j.neulet.2016.11.016

• Fetal alcohol syndrome disorder
• Zebrafish
• Glutamate
• Ethanol

Ethanol is a widely consumed substance throughout the world. During development it can substantially damage the human fetus, whereas the developing brain is particularly vulnerable. The brain damage induced by prenatal alcohol exposure may lead to a variety of long-lasting behavioral and neurochemical problems. However, there are no data concerning the effects of developmental ethanol exposure on the glutamatergic system, where extracellular glutamate acts as signaling molecule. Here we investigated the effect of ethanol exposure for 2 h (concentrations of 0.0%, 0.1%, 0.25%, 0.50%, and 1.00%) in embryos at 24 h post-fertilization (hpf) by measuring the functionality of glutamate transporters in the brain of adult (4 months) zebrafish. However, ethanol 0.1%, 0.25% and 0.50% decreased transport of glutamate to 81.96%, 60.65% and 45.91% respectively, when compared with the control group. Interestingly, 1.00% was able to inhibit the transport activity to 68.85%. In response to the embryonic alcohol exposure, we found impairment in the function of cerebral glutamate transport in adult fish, contributing to long-term alteration in the homeostasis glutamatergic signaling.

• 出版日期2017-1-1