Glycogen synthase kinase 3 beta regulation of nuclear factor of activated T-cells isoform c1 in the vascular smooth muscle cell response to injury

作者：Chow Winsion; Hou Guangpei; Bendeck Michelle P^*

来源：Experimental Cell Research, 2008, 314(16): 2919-2929.

DOI：10.1016/j.yexcr.2008.07.010

• atherosclerosis
• nuclear factor of activated T-cells
• smooth muscle cell
• migration
• proliferation
• glycogen synthase kinase 3 beta

The migration and proliferation of vascular smooth muscle cells (vSMCs) are critical events in neointima formation during atherosclerosis and restenosis. The transcription factor nuclear factor of activated T-cells-isoform cl (NFATc1) is regulated by atherogenic cytokines, and has been implicated in the migratory and proliferative responses of vSMCs through the regulation of gene expression. In T-cells, calcineurin de-phosphorylates NFATc], leading to its nuclear import, while glycogen synthase kinase 3 beta (GSK3 beta) phosphorylates NFATc1 and promotes its nuclear export. However, the relationship between NFATc1 and GSK3 beta has not been studied during SMC migration and proliferation. We investigated this by scrape wounding vSMCs in vitro, and studying wound repair. NFATc1 protein was transiently increased, reaching a peak at 8 h after wounding. Cell fractionation and immunocytochemistry revealed that NFATc1 accumulation in the nucleus was maximal at 4 h after injury, and this was coincident with a significant 9 fold increase in transcriptional activity. Silencing NFATc1 expression with siRNA or inhibition of NFAT with cyclosporin A (CsA) attenuated wound closure by vSMCs. Phospho-GSK3 beta (inactive) increased to a peak at 30 min after injury, preceding the nuclear accumulation of NFATc1. Overexpression of a constitutively active mutant of GSK3 beta delayed the nuclear accumulation of NFATc1, caused a 50% decrease in NFAT transcriptional activity, and attenuated vSMC wound repair. We conclude that NFATc1 promotes the vSMC response to injury, and that inhibition of GSK3 beta is required for the activation of NFAT during Wound repair.

• 出版日期2008-10-1