科研之友
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摘要
A simple, precise, rapid and accurate, binary-phase high performance liquid chromatographic method has been developed for the determination of arctiin and arctigenin contents in the Arctium tomentosum Mill. with short run time. Chromatographic separation was achieved by using HPLC system, consisting of a Shimadzu LC-6AD and Kromasil C(18) column (250x4.6 mm, 5 mu m, with pre-column), the mobile phase consists of methanol and water (55: 45). Detection wavelength was 280 nm. The speed of flow was 1.0 mL/min. The specimen handing quantity was 10 mu L. The arctiin's linearity range was 1.575 similar to 4.725 mu g (r=0.9995). The arctigenin's linearity range was 0.613, 3.063 mu g (r = 0.9998) and the linear relationship was accurate. The average recovery (n=5) of arctiin and arctigenin were 101.55% (RSD=2.23%). 101.63% (RSD = 1.49 %) respectively. The contents of arctiin and arctigenin in Arctium tomentosum Mill. were 10.69 mg/g and 0.15 mg/g, respectively. Therefore, the developed HPLC method can be applied to both in vitro studies of arctiin and arctigenin formulations as well as drug estimation in biological samples.
- 出版日期2011-12
- 单位新疆医科大学
