Apelin-13 increases expression of ATP-binding cassette transporter A1 via activating protein kinase C alpha signaling in THP-1 macrophage-derived foam cells

作者：Liu, Xiao-Yan; Lu, Qian; Ouyang, Xin-Ping; Tang, Shi-Lin; Zhao, Guo-Jun; Lv, Yun-Cheng; He, Ping-Ping; Kuang, Hai-Jun; Tang, Yan-Yan; Fu, Yuchang; Zhang, Da-Wei; Tang, Chao-Ke^*

来源：Atherosclerosis, 2013, 226(2): 398-407.

DOI：10.1016/j.atherosclerosis.2012.12.002

• Apelin
• ABCA1
• PKC alpha
• Calpain
• Atherosclerosis

Apelin has an antiatherogenic function through activating protein kinase C (PKC) to initiate a series of cellular signaling pathways. PKC phosphorylates and stabilizes ATP-binding cassette transporter A1 (ABCA1) through inhibiting its degradation mediated by calpain. Thus, in the present study, we investigated whether apelin-13 affects expression of ABCA1 through PKC signaling. The results showed that apelin-13 dramatically increased cholesterol efflux from THP-1 macrophage-derived foam cells and reduced cellular cholesterol levels. ABCA1 protein but not mRNA levels were dramatically increased by apelin-13, and calpain-induced degradation of ABCA1 and calpain activity were suppressed with treatment of apelin-13. However, the effects of apelin-13 on ABCA1 protein expression, cellular cholesterol efflux and calpain activity were abolished by depletion of PKC alpha, suggesting the potential important role of PKC alpha. In addition, apelin-13 was shown to phosphorylate serine residues in ABCA1 through the PKC alpha pathway. Thus, apelin-13 appears to activate PKC alpha, phosphorylate ABCA1 and inhibit calpain-mediated proteolysis, thereby promoting cholesterol efflux and reducing foam cell formation. Our study herein described a possible mechanism for understanding the antiatherogenic effects of apelin on attenuating the progression of atherosclerosis.

• 出版日期2013-2
• 单位南华大学