Antibiotic amphotericin B-DPPC lipid complex: X-ray diffraction and FTIR studies

作者：Kaminski Daniel M^*; Arczewska Marta; Pociecha Damian; Gorecka Ewa; Steniewski Andrzej; Gagos Mariusz

来源：Journal of Molecular Structure, 2015, 1080: 57-62.

DOI：10.1016/j.molstruc.2014.09.076

• Amphotericin B
• Multibilayers
• DPPC multibilayers
• X-ray diffraction

In this work, we studied the formation of a complex between the polyene antibiotic amphotericin B (AmB) and dipalmitoylphosphatidylcholine (DPPC) lipid multibilayers by means of X-ray diffraction and FTIR spectroscopy. The complex formation can be detected in multibilayers by characteristic reflection around 2.2 A(-1) . The FTIR spectra indicate that in this case AmB lowers the main phase transition of DPPC. Additionally, with an increase in temperature, a shift of AmB towards the hydrophilic part of the DPPC bilayer is observed. For the first time, such complex formation has been observed by Xray diffraction and discussed.

• 出版日期2015-1-25