Melanin biosynthesis inhibitory effects of calycosin-7-O-beta-D-glucoside isolated from astragalus (Astragalus membranaceus)

作者：Kim Jin Hee; Lee Eun Sook; Lee Choong Hwan^*

来源：Food Science and Biotechnology, 2011, 20(6): 1481-1485.

DOI：10.1007/s10068-011-0205-4

• Astragalus membranaceus
• calycosin-7-O-beta-D-glucoside
• tyrosinase
• melanin biosynthesis

To discover an active skin depigmentation agent, we isolated a melanin biosynthesis inhibitor from the methanol extract of astragalus (Astragalus membranaceus) using a bioactivity-guided fractionation and identified it as calycosin-7-O-beta-d-glucoside via spectroscopic analysis. The active compound exhibited tyrosinase inhibitory activity with an IC(50) value of 68 mu M. In addition, calycosin-7-O-beta-d-glucoside showed a melanin biosynthesis inhibition zone in a culture plate of Streptomyces bikiniensis. Furthermore, 75.78 mu M of calycosin-7-O-beta-d-glucoside dramatically decreased 50% of the melanin content on Melan-a cells without any apparent cytotoxicity. Theses results indicate that the calycosin-7-O-beta-d-glucoside isolated from astragalus may be a good candidate for skin-whitening agent.

• 出版日期2011-12